Alchemist's Assistant Wish (Plasmium Locations)
The Alchemist’s Assistant is a Gather Wish in Hollow Knight: Silksong. You are tasked by the eccentric Alchemist Zylotol to venture into the winding Wormways and collect samples from three rare Plasmium Plants to help him complete his experiment.
Phase 1: How to Start the Quest
Section titled “Phase 1: How to Start the Quest”-
Find Zylotol’s Lab Travel to the Wormways. In the upper-left area of the region, you will find the unique laboratory of Alchemist Zylotol. There is a bench inside, making it a convenient landmark.

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Accept the Wish Speak with Zylotol. He will complain about his prior assistant and offer you the job. Accept his wish to collect three Plasmium buds.

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Equip the Needle Phial Zylotol will give you a temporary tool, the Needle Phial, specifically for this quest. You must sit at the bench in his lab and equip it in one of your tool slots to begin harvesting.
Phase 2: All Plasmium Plant Locations
Section titled “Phase 2: All Plasmium Plant Locations”The Wormways can be confusing to navigate. This guide provides a sequential, step-by-step path from one plant to the next.

Plasmium Plant 1: Below the Lab
Section titled “Plasmium Plant 1: Below the Lab”-
Exit Zylotol’s lab to the right and drop down into the level below.
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Turn left and use your Wall Climb ability over the spike pit to reach a platform.
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Go left again to drop down. You will see the blue glow of the first Plasmium node in an alcove to your right.
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Approach the plant, then press and hold your Tool button to charge the Needle Phial. Release to strike the plant and collect the Plasmium.



Plasmium Plant 2: The Mossy Tunnel
Section titled “Plasmium Plant 2: The Mossy Tunnel”-
From the first Plasmium’s location, turn left and drop down to the platform below.
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Jump left and take the drop leading down to the next level.
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Drop to the platform on the left, then head right to find another hole leading even further down.
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Go right to drop down again, then turn left for another drop with a spike pit at the bottom.
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Drop down to the right, and you will find a moss-covered tunnel. The second Plasmium node is at the end.


Plasmium Plant 3: The Hidden Cavern
Section titled “Plasmium Plant 3: The Hidden Cavern”-
From the second Plasmium’s location, turn left and use Wall Climb to start heading back up the way you came.
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To the left of the first falling sandworm, you’ll see a spike pit with a wall on the other side. Wall climb and jump across the gap to the left.
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Keep heading left until you see another drop. Fall down and immediately turn left mid-air to find an alcove containing a breakable stone wall.
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Break the wall and follow the hidden tunnel to the left to find the third and final Plasmium node.



Phase 3: Completing the Quest
Section titled “Phase 3: Completing the Quest”-
Return to Zylotol Once you have collected all three Plasmiums, the quest will update. Make your way back to Alchemist Zylotol’s lab.

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Turn in the Quest Speak with Zylotol to hand over the samples. He will be pleased with your work and give you your reward.

Wish Reward: Plasmium Phial Tool
Section titled “Wish Reward: Plasmium Phial Tool”For your efforts, Zylotol gives you a permanent, refillable version of the phial.

The Plasmium Phial is a powerful Tool with the following mechanics:
- Effect: When used, it grants Hornet temporary extra health masks, similar to Lifeblood. Each use grants 3 temporary masks.
- Charges: It comes with 3 active charges and 20 charges in reserve.
- Refilling: Resting at a bench automatically refills the active charges from your reserve, consuming some Shell Shards in the process.
- Zylotol’s Service: You can return to Zylotol to fully refill your reserve charges for a flat fee of 50 Rosaries.
- Synergy: The concoction can be mixed with other Tools to create different effects.
Quest Overview